A method is described for the preparation of Ca 2+-resistant myocytes from the adult xhustler.xyz 2+-resistant cells were obtained in yields of ± × 10 6 cells/gm wet weight tissue, with a purity of ± % cells excluding trypan blue. Of the cells which excluded trypan blue, over 90% were rod-shaped. The rodshaped cells when isolated were quiescent, but beat in response to . Abstract Objective: To compare adult rat cardiomyocytes in primary culture for up to 28 days with those in primary culture for 10 days plus up to 18 days in a coculture (CC) system.
Structural and functional properties of adult rat heart myocytes lysed with digitonin. J Biol Chem. Nov 15; (26)– Wenger WC, Murphy MP, Kindig OR, Capen CC, Brierley GP, Altschuld RA. Mitochondrial enzyme retention by irreversibly damaged rectangular isolated adult rat heart myocytes. Calcium-tolerant adult rat ventricular myocytes (ARVMs) were obtained from hearts of male Sprague-Dawley rats ( to g) as previously described. 18 Briefly, animals were anesthetized with sodium pentobarbital (50 mg/kg IP) and heparinized ( USP/kg IV), and their hearts were aseptically removed into an ice-cold modified cardioplegic solution (KB .
Transformation of adult rat cardiac myocytes in primary culture. Banyasz T(1), Lozinskiy I, Payne CE, Edelmann S, Norton B, Chen B, Chen-Izu Y, Izu LT, Balke CW. Author information: (1)University of Kentucky, South Limestone Street, Lexington, KY , USA. [email protected] Isolation of Ventricular Myocytes from Adult Rat. Race L. Kao, Ph.D. East Tennessee State University Johnson City, TN Email: [email protected] Introduction: Isolated cardiac myocytes from adult mammals have been used for metabolic, electrophysiologic, receptor and ligand binding, pharmacologic, and recently cellular cardiomyoplasty studies.